Project:

DNA Barcoding of Peconic River Aquatic Macroinvertebrates

Students:

Kainoa Bosch, Matthew Keller, Jeffery Smith, John Palina

School:

Eastport South Manor Junior-Senior High School, Suffolk

Mentors:

Robert Bolen

Abstract:

The Peconic Estuary houses many different plants, animals, and insects. Humans depend heavily on the estuary, as it’s important for our economy and recreation. However, these organisms are in danger because of multiple threats including: The brown tide algae blooms, nitrate levels, contamination (or runoff) of the water and invasive species. According to the National Geographic Society, invasive species overpopulate very fast and compete with native species for food. Some invasive species of the Peconic River include hard clams and Asian clams. Indicator species indicate the overall quality of the water and environment. Dragonfly nymphs are indicators of good water quality and leeches are indicators of poor water quality. Barcoding helps identify organisms based upon genetic differences. When barcoding, the CO1 gene is amplified. This gene is found in the mitochondria and can evolve rapidly, allowing scientists to see differences in organisms. An ecosystem's stability depends on its

Poster:

Team samples: