Spiders play a big role in biodiversity and are important to our ecosystems. They usually appear during summer to fall. Without spiders, the food chains all living things go through will no longer exist and it would cause a big gap in our ecosystem. Studying biodiversity helps conserves, saves species from becoming extinct and control ecological systems. Biodiversity spiders can be achieved by DNA barcoding and taxonomic research on the cobwebs of spiders. DNA Barcoding leads to the increased knowledge of spider species and the preys they target. DNA extraction from cobwebs is a safe method in determining diversity of spiders without even harming the spiders.