Species models are not widely agreed upon. Due to the availability of gene sequences, there have recently been more discoveries that reveal there may be more species than previously thought in various taxa. DNA barcodes are an effective way to distinguish between various species, but interspecific genetic distance is not always consistent across species. Intraspecific distance isn’t consistent either. We would like to explore this variation utilizing data from GenBank of sister species at the Isthmus of Panama. We would use published COI sequences of sister species from the Acar subgenus, and compare the genetic distance between and within two species.