Microscopic aquatic invertebrates can be found in all bodies of water that are within the islands boundaries (5). The specific aim of this project is to collect and determine species of microscopic aquatic invertebrates in two different locations, Frog Pond and Fresh Pond, around Shelter Island and determine the difference in diversity among the two ponds. A microscopic seine net will be used to pick up the invertebrates in order to extract their DNA and identify the species they belong to. The objective is to find the difference in the variety of organisms between two different sizes of freshwater ponds on Shelter Island.