Symposium: 2018 |2017 | 2016 | 2015 | 2014
The fifth year of the Urban Barcode Research Program concluded on May 24 with poster sessions at the New York Academy of Medicine, New York.
At a Glance:
20 participating teams
40 students from 26 high schools
20 scientists from 11 institutions
Taxonomic Group Studied:
Students collected 500+ samples, an average of 25 samples per team. Traditional DNA barcoding methods produced 2500+ sequence reads, and next-generation sequencing methods generated more than 100 million sequence reads.
Elizabeth Alter, Ph.D.
Assistant Professor
Marine Evolutionary & Ecological Genetics
York College and The Graduate Center, CUNY
Elizabeth Argiro and Joshua Antony
Joshua Antony, Regis High School, and Elizabeth Argiro, Hunter College High School. Mentored by Benajmin tenOever, Icahn School of Medicine at Mount Sinai.
In an effort to survey and isolate novel viruses, students sequenced RNA from a collection of arthropods found in the New York area. Students identified and named the novel Jiminy Cricket virus. The virus is composed of a positive-sense single-stranded RNA that encodes four putative open reading frames (ORF1-ORF4) and is related to members of the Negevirus group. RNA transfection of Jiminy Cricket virus into arthropod and mammalian cultures demonstrated limited replication only in arthropod cells, which is consistent with members of this virus group.
Edward Myers, Nicole Kanzler, and Henry Kates
Henry Kates, Trinity School, and Nicole Kanzler, Baruch College Campus High School. Mentored by Edward Myers, American Museum of Natural History
Crotalus cerastes is a species of rattlesnake native to the southwestern United States and northern Mexico. Three subspecies have been described, Crotalus cerastes cerastes, Crotalus cerastes cercobombus, and Crotalus cerastes laterorepens. However, subspecies are often classified based on color pattern and other superficial traits that may not represent the evolutionary history of the species. By obtaining DNA from samples of Crotalus cerastes and barcoding them, the students determined the genetic diversity of populations at varying levels of genetic and geographic interconnectedness. The findings suggested that both within the same subspecies and within the same general geographical area, there were significant enough genetic differences that distinct genetic groups could be identified. This implies that the way organisms are grouped into subspecies based on morphology may not be indicative of evolutionary history.
The fourth year of the Urban Barcode Research Program concluded on May 25 with poster sessions at the New York Academy of Medicine, New York.
At a Glance:
19 participating teams
39 students from 26 high schools
18 scientists from 15 institutions
Taxonomic Group Studied:
Students collected 600+ samples—an average 32 samples per team. Traditional DNA barcoding methods produced 1000+ sequence reads, and next-generation sequencing methods generated more than a million sequence reads.
Jesse H. Ausubel
Director, Program for the Human Environment
The Rockefeller University
This project focused on building a system of DNA barcodes for the fish species in the Bronx River for further environmental research. We collected 11 samples and used two specific primer sets to target two mitochondrial loci, one on the COI gene and one in the 12S locus to amplify the DNA of our samples. 10 out of 11 samples were sent to sequence. However, only 6 samples had high quality DNA sequences that can be used for identification. This project can benefit scientists interested in exploring the fish species or the biodiversity in the Bronx River. Our follow-up project focuses on completing the DNA barcode data for the all fishes in the Bronx River and reporting new DNA sequences to the NCBI database.
The third year of the Urban Barcode Research Program concluded on June 3, 2016 with poster sessions at the Borough of Manhattan Community College, New York.
At a Glance:
20 participating teams
42 students from 24 high schools
19 scientists from 13 institutions
Taxonomic Group Studied:
Students submitted 700+ samples for sequencing —an average 35 samples/team. Traditional DNA barcoding methods produced 1000+ sequence reads, and next-generation sequencing methods generated 1 million+ sequence reads.
Pamela Stavrakos and Katherine Lamattina mentored by Eugenia Naro-Maciel, Seth Wollney, and John Davis
The major aims of this study are to 1) characterize aquatic invertebrate biodiversity in NYC, and 2) to compare and contrast DNA barcoding and eDNA metabarcoding approaches for revealing an ecosystem’s taxonomic profile. Additionally, we hope to 3) build a library of local genetic variants for Staten Island.
Diatoms have been used in forensic investigations to determine if someone died from drowning. Our aim is to find out the most abundant diatoms at one location of New York City waterways. The results of this study can be potentially helpful in drowning investigations by law enforcement agencies.
Thanks to all of the teams who submitted proposals, performed experiments, and presented project results at the poster sessions held at the Linder Theater, American Museum of Natural History on June 15, 2015.
At a Glance:
20 participating teams
39 students from 21 high schools
17 scientists from 10 institutions
Taxonomic Group Studied:
Students submitted 600+ samples for sequencing – an average 30 samples/team. Traditional DNA barcoding methods produced 800+ sequence reads, and next-generation sequencing methods generated 1 million+ sequence reads.
Thanks to all of the teams who submitted proposals, performed experiments, and presented project results at the poster sessions held at Kathryn W. Davis Teaching Classroom, American Museum of Natural History on April 29, 2014.
20 participating teams
40 students from 17 high schools
16 public and 1 private
19 scientists from 12 institutions
Taxonomic Group Studied:
Students submitted 500+ samples for sequencing – an average 27 samples/team. Traditional DNA barcoding methods produced 800+ sequence reads, and next-generation sequencing methods generated 600,000+ sequence reads.