For each group
*Store on ice
Avoid pouring an overly thick gel, which makes visualization of the DNA more difficult.
The gel will become cloudy as it solidifies.
Do not add more buffer than necessary. Too much buffer above the gel channels electrical current over the gel, increasing running time.
Expel any air from the tip before loading, and be careful not to push the pipette through the bottom of the sample well.
Transillumination, where the light source is below the gel, increases brightness and contrast.