This project will barcode a variety of fungi collected at Brookside County park to confirm samples to the lowest possible taxonomic level since keys are difficult to accurately identify fungi. Additionally, species richness and smoothness of fungi collected within the palustrine forested area of Brookside County park will be calculated to assess mushroom biodiversity at the park for this habitat type. Lastly, sequence results will be compared to those in genbank to identify novel barcodes using DNA Subway bioinformatic tools (Blue Line).